Advantages of PB-NK Cells Synthetic NK Cells - Advantages of PB-NK Cells - Technology

Comparison of different resources of allo-NK cells：PB-NK cells are most effective

Autologous Blood-derived NK cells:

hard collection since patients’ status sometimes and weak anti-tumor efficacy since suppression by tumor burden.
Donor Blood-derived NK cells:

NK cells undergoing complete in vivo development and functional maturation and extensive donor/recipient pairing; potent anti-tumor efficacy; suitable for “off-the-shelf” production.
iPSC-derived NK cells:

homogeneity and easier genetic manipulation and “off-the-shelf” production; immature functionality (lack of entire NK receptor array such as without CD16, KIR and donor/recipient pairing); potential of malignant transformation.
Cord blood-derived NK cells:

higher in-vivo proliferation, high NK cell concentration (15–30%); immature NK cells with lower cytotoxicity.
NK 92 Cell line:

derived from a patient with NK lymphoma; need for irradiation before infusion; limited in vivo activity; safety concern due to tumorigenicity.

Functional comparison of different resources of allo-NK cells：Donor PB-NK with Entire Functionality only after Complete Development in Human Body

Phenotype	PB-NK	iPSC-NK
KIRs/NKRs(Recognizing tumor)	+ + + +	+
CD16 (ADCC effect)	+ + + +	+
CD62L(Immigration to tumor sites)	+ + + +	+

History of NK CellTech Investigator Using Allo-NK Cells in Adoptive Cellular Immunotherapy with 30 years’ experience

“The therapeutic efficacy of allogeneic blood-derived NK cells in 38 patients with advanced solid tumor” reported at 1994 in a Chinese journal (official journal of China Anti-Cancer Association)
After observation of clinical efficacy of allo-NK cells in treating solid tumor in mainland China, Dr.Tian moved to National Cancer Institute (NCI) to further confirm this in animal model which published at 1998.

CAR-NK VS. CAR-T：much safer with no MHC restriction and improved efficacy

Limitations of CAR-T cell Therapy
- Mostly autologous cells
- High cost in manufacturing
- Potential toxicity (CRS and neurotoxicity)
- must be hospitalized with ICU support
Advantages of CAR-NK cell Therapy
- A variety of allogeneic cell sources
- Low cost in manufacturing
- Without hospitalization owing to the lack of CRS and/or neurotoxicity
- off-the-shelf’ products for multiple outpatients